How to Dispense Small Volume Liquids With Precision

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Bulk microplate-based liquid dispensing instruments typically use peristaltic pumps or microprocessor- controlled syringe pumps to perform reagent and cell dispensing. Each pump type has advantages and disadvantages depending on the reagent used and the lab's needs. Liquid dispensing syringe pump must often choose between the technologies, thus limiting their assay choice. If they liquid dispensing syringe pump both types, labs often incur liquid dispensing syringe pump expense and reduced lab space to purchase multiple instruments.

The modular design means that users may purchase one dispensing type now and add the other dispensing types in the future when assay requirements change. The peristaltic pump technology uses flexible tubing and rollers affixed to a rotor that compresses the tubing, containing fluid, at regular intervals to force out a measured liquid dose.

The dose volume is dependent on the roller distance, tubing tension, internal diameter and dispenser tip geometry. Advantages of peristaltic pump dispensing are: Additionally, low prime volumes and the ability to back-flush conserve precious reagents and cells. However, the disadvantages of peristaltic pump dispensing include reduced compatibility with high viscosities and fractional rather than full volume dispensing. Recalibration and replacement tubing also add to the long-term instrument cost.

Syringe dispensers are controlled by microprocessors and feature a faster output than mechanical peristaltic pump dispensers along with high liquid dispensing syringe pump and precision. Additionally, there is no fluid output pulse and the flow rate is fully controllableā€”from gentle cell dispensing to vigorous reagent dispensing.

Syringe dispensers do not require consumables or routine calibration, thus making them particularly well suited for high throughput batch processing workflows. Disadvantages of syringe dispensing are that more prime volume is required and the volume range may be more limiting than the range offered by peristaltic pump dispensers. A bulk liquid dispenser offering peristaltic pump and syringe dispensing in one unit, coupled with automation and stacking compatibility, provides increased assay flexibility and throughput and saves valuable bench space.

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Stop Drowning in Paper! By BioTek April 06, For more information, visit www. Linda helps her colleague liquid dispensing syringe pump his pipetting liquid dispensing syringe pump, with exciting results!

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